Qscore by considering the amplification efficiency, mapping sensitivity, precision on multi-tier taxonomic annotation, sequence type and length. The Qscore ranges from 0% to 100%, and higher value indicates better overall performance.

16S rRNA gene amplicon sequences can be picked into OTUs against reference by Parallel-META Suite. With a sequence file “sample1.fa”, you can get the OTUs using Parallel-META Suite by

$PM-parallel-meta -r sample1.fa -o sample1.out -f F

Then in the output directory "sample1.out", the result file "classification.txt" is qualified for upload and search.

The query file contains two columns: the OTUs and their sequence counts.

Example: